C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/81 (2006.01) C12N 1/19 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2384123
Transcription promoter and terminator sequences from the Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase 2 gene (GAP2 gene) are disclosed. The sequences are useful within DNA constructs for the production of proteins of interest in cultures P. methanolica cells. Within the expression vectors, a GAP2 promoter and/or a GAP2 terminator is operably linked to a DNA segment encoding the protein of interest.
L'invention concerne des séquences de promoteur de transcription et de terminateur utilisées dans le gène de glycéraldéhyde-3-phosphate déshydrogénase 2 (gène GAP2) issues de Pichia methanolica. Ces séquences sont utiles dans des constructions d'ADN permettant de produire des protéines considérées dans des cellules cultivées de P. methanolica. Dans les vecteurs d'expression, un promoteur et/ou un terminateur de GAP2 est lié, de manière fonctionnelle, à un segment d'ADN codant pour la protéine considérée.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Zymogenetics Inc.
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