C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2753737
The present invention provides a primer extension reaction method, such as a PCR method, for structure-independent amplification of DNA containing CG-rich repeat sequences wherein in the extension step the temperature fluctuates between a first extension temperature and a second extension temperature. The present invention also provides methods for diagnosing disorders. The present invention also provides a thermal cycler programmed to perform the method of the invention.
La présente invention concerne un procédé de réaction par extension damorce, tel quun procédé PCR, pour lamplification indépendante de la structure dADN contenant des séquences de répétition riches en CG, la température fluctuant entre une première température dextension et une seconde température dextension pendant létape dextension. La présente invention concerne également des procédés de diagnostic de troubles. La présente invention concerne également un cycleur thermique programmé pour réaliser le procédé selon linvention.
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