Reagent used for determining factor viii activity

Details Reagent used for determining factor viii activity Reagent used for determining factor viii activity

G - Physics

01

N

G01N 33/68 (2006.01) C12Q 1/56 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2059605

ABSTRACT OF THE DISCLOSURE: To determine the factor VIII activity of a sample, the latter is reacted with a reagent containing thrombin, factor IXaB, phospholipids and calcium ions so as to form a factor VIIIa-factor IXa-phospholipid-calicum ion-containing complex. This complex is reacted with factor X so as to obtain activated factor X. The obtained factor Xa is reacted with chromogenic substrate so as to form a substance to be determined spectrophotometrically. Complex formation and activation of factor X are effected in one stage: The sample is mixed with a mixture of human thrombin, phospholipids, calcium ions, human factor IXaB, human factor X and, if desired, human factor XIa and human factor XIIa, as a complex-forming reagent.

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