Combined exponential and linear amplification

Details Combined exponential and linear amplification Combined exponential and linear amplification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2504198

Methods and compositions are provided for sensitive detection and quantitation of nucleic acids. Methods and compositions are further provided for genotyping. Probes of the invention allow target initiated amplification for single stranded, double stranded polynucleotides and pyrophosphate (PPi). DNA enzyme mediated detection method is also provided for detecting single stranded end products.

L'invention concerne des procédés et des compositions permettant une détection sensible et une quantification d'acides nucléiques. L'invention concerne en outre des procédés et des compositions adaptées au génotypage. Les échantillons de l'invention permettent une amplification à initiation de cible pour des polynucléotides à un seul brin, à double brin et du pyrophophate (PPi). L'invention concerne également la méthode de détection induite par enzyme ADN, pour détecter des produits terminaux à un seul brin.

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