Homogenising of small-volume mixtures by centrifugation and...

B - Operations – Transporting – 01 – J

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B01J 19/28 (2006.01) B01F 3/08 (2006.01) B01F 9/00 (2006.01) B01F 15/06 (2006.01) B01J 19/08 (2006.01) B01L 7/00 (2006.01) B04B 5/02 (2006.01) B04B 5/10 (2006.01) B04B 15/02 (2006.01) C12M 1/36 (2006.01) C12M 1/38 (2006.01) C12M 1/40 (2006.01) G01N 1/38 (2006.01) G01N 1/44 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2366732

The efficient temperation of a sample is achieved when the sample is subjected to centrifuga-tion simultaneously with heating and optionally also cooling. This is achieved in a device compris-ing at least a rotor (1) for hold-ing reaction vessels (2), a motor (3), connected to said rotor, means such as a processor (4) for controlling the speed of the ro-tor, and means (5 and 6) for heat-ing and cooling the contents of the reaction vessels, wherein the means (6) for heating cover the apices of the reaction vessels for at least part of the rotational path of said vessels and that the means for heating operate at a temperature significantly higher than the melting temperature of the reaction vessels. The invention further relates to a method for performing chemical reactions and in particular a method for performing biochemical reactions involving thermocycling, for example PCR-reactions.

La présente invention concerne un procédé permettant de réaliser avec efficacité l'homogénéisation d'un échantillon qui est soumis à centrifugation en même temps qu'à un réchauffage et éventuellement aussi à un refroidissement. A cet effet, on utilise un dispositif comportant au moins un rotor (1) servant de support aux cuves de réaction (2), un moteur (3) relié au rotor, des organes tels qu'un processeur (4) pour réguler la vitesse du rotor, et des organes (5, 6) permettant de réchauffer et refroidir le contenu des cuves de réaction. De fait, les réchauffeurs (6) recouvrent au moins les sommets des cuves de réaction sur au moins une partie du chemin de rotation de ces cuves. En outre, les réchauffeurs opèrent à une température largement supérieure à la température de fusion des cuves de réaction. L'invention concerne également un procédé permettant de mener des réactions chimiques, et plus particulièrement de mener des réactions biochimiques impliquant un thermocyclage, notamment des réactions d'amplification en chaîne par polymérase.

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