Primers for amplification and sequencing of eubacterial 16s...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2601658

The invention relates to oligonucleotides for the qualitative and/or quantitative amplification and/or the sequencing of 16S rDNA-genes, as well as of fragments thereof and RNA derived thereof. It relates to their use as primers in amplification reactions and in sequencing, in particular in combination for the identification of the genus/species/strain of the bacterial sample or clinical isolate.

L'invention concerne des oligonucléotides pour l'amplification qualitative et/ou quantitative et/ou le séquençage de gènes d'ADNr 16S, ainsi que des fragments de ces derniers et un ARN dérivé de ces derniers. L'invention concerne leur utilisation en tant qu'amorces dans des réactions d'amplification et dans le séquençage, en particulier en combinaison en vue de l'identification du genre/de l'espèce/de la souche de l'échantillon bactérien ou de l'isolat clinique.

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