A - Human Necessities – 01 – N
Patent
A - Human Necessities
01
N
A01N 1/02 (2006.01) A61L 27/36 (2006.01)
Patent
CA 2103025
A method is disclosed for preparing a transplantable tissue which has been cryopreserved with an intracellular cryoprotect- ant and then thawed. After thawing, the tissue is treated with a diluent (or eluent) solution to reduce the level of cryoprotectant in the cells to a substantially non-toxic level. This is conducted using a single dilution step. Cryoprotectants which are removable in this manner include dimethylsulfoxide, glycerol, propanediol and other compounds which penetrate the cells. The dilution step does not require end point titration and can be conducted over a time period greater than about 5 minutes.
Brockbank Kelvin G. M.
Carpenter John F.
Cryolife Inc.
Smart & Biggar
LandOfFree
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