Method of forming dendritic cells from embryonic stem cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/0797 (2010.01) C12N 5/0735 (2010.01) C12N 5/0784 (2010.01) A61K 39/00 (2006.01)

Patent

CA 2610243

This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.

L'invention concerne la culture de cellules dendritiques à partir de cellules souches embryonnaires humaines (ES). Ces cellules ES sont d'abord cultivées dans des cellules hématopoïétiques par co-culture avec des cellules stromales. Des cellules différentiées dans la lignée hématopoïétique sont ensuite cultivées avec GM-CSF afin de créer une culture de cellules précurseurs myéloïdes. La culture de ces cellules précurseurs myéloïdes avec les cytokines GM-CSF et IL-4 provoque la génération de cellules dendritiques fonctionnelles. Ces cellules dendritiques possèdent un phénotype unique, tel qu'il est indiqué par leur combinaison de marqueurs de surface.

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