Detection of biological threat agents

Details Detection of biological threat agents Detection of biological threat agents

G - Physics

01

N

G01N 33/543 (2006.01) C12N 1/00 (2006.01) C12Q 1/68 (2006.01) C25B 1/00 (2006.01) C25B 9/04 (2006.01) G01N 25/22 (2006.01) G01N 27/00 (2006.01) G01N 27/416 (2006.01) G01N 33/53 (2006.01) G01N 33/553 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2503163

The technical features mentioned in the abstract do not include a reference sign between parentheses (PCT Rule 8.1(d)). Methods and devices are provided for detecting and confirming the identity of analytes such as threat agents, particularly analytes which are air or fluid borne. The methods comprise providing an affinity reagent having a known charge density in a medium which is capable of conducting electrical current, and the devices comprise a sensor as depicted in the figure below. Figure 4 (a) depicts the probe module portion of the sensor, comprising a bulb (26), delivery tubes (27) and probe chambers (28), and is connected to the power sources (batteries, 24) and the LED (designated "L", 25). The chip (29) comprising an array of discrete transistors (30) is connected to the probe module to enable deployment of the probe reagents to the transistor array. Analyte identity is probed by manipulation of the charge density of the affinity reagent~analyte complex, including methods comprising altering probe conditions for different pH solutions, charge, a secondary affinity reagent, or combinations thereof. Methods and devices are provided for detecting and confirming the identity of analytes such as threat agents, particularly analytes which are air or fluid borne. The methods comprise providing an affinity reagent having a known charge density in a medium which is capable of conducting electrical current, and the devices comprise a sensor as depicted in the figure below. Figure 4 (a) depicts the probe module portion of the sensor, comprising a bulb (26), delivery tubes (27) and probe chambers (28), and is connected to the power sources (batteries, 24) and the LED (designated "L", 25). The chip (29) comprising an array of discrete transistors (30) is connected to the probe module to enable deployment of the probe reagents to the transistor array. Analyte identity is probed by manipulation of the charge density of the affinity reagent-analyte complex, including methods comprising altering probe conditions for different pH solutions, charge, a secondary affinity reagent, or combinations thereof.

L'invention concerne des procédés et des dispositifs de détection et de confirmation de l'identité d'analytes tels que des agents de menace, en particulier des analytes portés dans l'air ou dans un fluide. Les procédés consistent à fournir un réactif d'affinité ayant une densité de charge connue dans un milieu capable de conduire le courant électrique. L'identité de l'analyte est sondée par une manipulation régulée de la densité de charge du complexe réactif d'affinité-analyte, à l'aide d'une variété de procédés.

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