Process for producing mammals with defined genetic properties

Details Process for producing mammals with defined genetic properties Process for producing mammals with defined genetic properties

C - Chemistry, Metallurgy

12

N

C12N 15/89 (2006.01) A01K 67/027 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2263729

The invention pertains to a process for developing mammals with definite genetic features, including transgenic mammals. In order to obtain in one single step animals entirely derived from ES cells (embryonic stem cells), in vitro genetically engineered and cultured totipotent cells (embryonic stem cells or embryonic germ cells) are injected into tetraploid blastocysts, and the resulting embryo is implanted into a surrogate mother.

L'invention concerne un procédé d'obtention de mammifères présentant des propriétés génétiques définies, notamment des mammifères transgéniques. Afin d'obtenir dans une première étape de traitement des animaux entièrement dérivés de cellules ES (cellules souches embryonnaires), des cellules totipotentes manipulées génétiquement in vitro et mises en culture (cellules souches embryonnaires ou cellules germinales embryonnaires) sont injectées dans des blastocystes tétraploïdes, et l'embryon qui en résulte est introduit dans une mère porteuse.

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