C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.2, 195/1.2
C12N 15/56 (2006.01) C12N 1/19 (2006.01) C12N 9/36 (2006.01) C12N 15/00 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2002480
Yeast cells whose DNA has been appropriately engineered to produce and to secrete lysozyme under control of a regulatable promoter are grown in the substantial absence of lysozyme synthesis and then are induced to produce and to secrete lysozyme under growth-limited conditions. This process is particularly suitable for the production of human lysozyme or mutants thereof.
Des cellules de levure, dont l'ADN a été adéquatement modifié pour produire et sécréter le lyzozyme sous le contrôle d'un promoteur que l'on peut contrôler, sont cultivées dans un milieu réprimant essentiellement la synthèse du lyzozyme, puis sont induites à le produire et à le sécréter dans des conditions limitées de croissance. Ce procédé convient particulièrement à la production du lyzozyme humain ou de ses mutants.
de Baetselier Annie
Hanotier Jacques D.v.
Rosenberg Steven
Fina Research S.a.
Gowling Lafleur Henderson Llp
Novartis Vaccines And Diagnostics Inc.
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