Methodology for colorimetrically determining the...

Details Methodology for colorimetrically determining the... Methodology for colorimetrically determining the...

C - Chemistry, Metallurgy

12

Q

C12Q 1/44 (2006.01) C12M 1/40 (2006.01) C12Q 1/37 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2161574

A composition. method and test device for determining the presence of leukocytes, esterase or protease in a test sample are disclosed. The composition comprises an ester which is hydrolyzed in the presence of leukocyte. esterase or protease to form a reaction product which couples with a diazonium salt to produce a detectable color change. The composition further comprises the salt of an alkaline earth metal which stabilizes the composition during manufacture and prevents the occurrence of false or background color change due to reactivity of the diazonium salt in the absence of leukocyte, esterase or protease.

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