C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) A01K 67/00 (2006.01) C07K 14/435 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12Q 1/00 (2006.01) C12Q 1/37 (2006.01) C12Q 1/68 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2415458
The invention relates to an autofluorescent fusion protein which is suitable for use as a protease substrate. The invention further relates to a nucleic acid sequence coding for said fusion protein, in addition to a method using the fusion protein and/or the nucleic acid sequence in a two coloured, confocal fluorometric assay for the detection and quantification of proteolytic activity in liquid samples or cells.
L'invention concerne une protéine hybride autofluorescente, appropriée pour servir de substrat de protéase. L'invention concerne également une séquence d'acides nucléiques codant cette protéine hybride, ainsi qu'un procédé faisant appel à cette protéine hybride et/ou à cette séquence d'acides nucléiques dans un test confocal fluorimétrique bichrome servant à mettre en évidence et à quantifier une activité protéolytique dans des échantillons liquides et des cellules.
Kettling Ulrich
Koltermann Andre
Kuhlemann Rene
Schwille Petra
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Direvo Biotech Ag
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