C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/72 (2006.01)
Patent
CA 2700555
The present invention relates to an efficient and improved process for purifying a recombinant protein. The invention relates to the purification of tissue plasminogen activator (tPA), such as truncated human tPA, recombinantly produced in bacteria, for example in E, coli. The present invention provides a process that requires less refolding; volume after solubilization of inclusion bodies isolated from cells expressing the recombinant tPA, without affecting the yield and purity of the tPA protein. The invention also provides optimum arginine concentrations for use during protein refolding and during ion exchange chromatography.
L'invention concerne un procédé efficace et amélioré de purification d'une protéine recombinante. L'invention concerne plus particulièrement la purification d'un activateur tissulaire du plasminogène (tPA), tel qu'un tPA humain tronqué, produit par recombinaison dans des bactéries, E. coli par exemple. L'invention concerne également un procédé nécessitant moins de repliements après la solubilisation de corps d'inclusion isolés à partir de cellules exprimant le tPA recombinant, mais n'affectant pas le rendement ni la pureté de la protéine tPA. L'invention concerne encore des concentrations optimales d'arginine destinées à être utilisées pendant le repliement de protéine et pendant la chromatographie par échange d'ions.
Bhavesh Saxena
Gita Arvind
Sudip Kumar Majumder
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Reliance Life Sciences Pvt. Ltd.
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