Microbial strains and processes for the manufacture of...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/21 (2006.01) C12N 1/08 (2006.01) C12N 9/22 (2006.01) C12P 7/62 (2006.01) C12P 19/00 (2006.01)

Patent

CA 2325350

DNA constructs and genetically engineered microbial strains constructed using these DNA constructs, which produce a nuclease enzyme with specificity for DNA and/or RNA, are provided. These strains secrete nuclease into the periplasm or growth medium in an amount effective to enhance productivity and/or recovery of polymer, and are particularly suited for use in high cell density fermentation processes. These constructs are useful for modifying microbial strains to improve production and recovery processes for polymers such as intracellular proteins, such as enzymes, growth factors, and cytokines; for producing polyhydroxyalkanoates; and for producing extracellular polysaccharides, such as xanthan gum, alginates, gellan gum, zooglan, hyaluronic acid and microbial cellulose.

Cette invention a trait à des produits de recombinaison d'ADN ainsi qu'à des souches microbiennes assemblées par génie génétique, obtenues en utilisant ces produits de recombinaison d'ADN, et qui produisent l'enzyme nucléase dotée d'une spécificité pour l'ADN et/ou l'ARN. Ces souches, qui sécrètent la nucléase dans le périplasme ou un milieu de croissance sous une quantité propre à accroître la productivité et/ou le recouvrement de polymères, conviennent du mieux à des processus de fermentation à densité cellulaire élevée. On utilise ces produits de recombinaison d'ADN pour modifier des souches microbiennes afin d'améliorer la production et le recouvrement de polymères, notamment des protéines intracellulaires, des enzymes, des facteurs de croissance et des cytokines, pour produire des polyhydroxyalcanoates ainsi que pour produire des polysaccharides extracellulaires, comme de la gomme de xanthane, des alginates, de la gomme gellane, du zooglan, de l'acide hyaluronique et de la cellulose microbienne.

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