C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/44 (2006.01) C07D 213/61 (2006.01) C07D 277/32 (2006.01) C07D 401/06 (2006.01) C07D 417/06 (2006.01) C07K 16/00 (2006.01) G01N 33/543 (2006.01) A61K 39/385 (2006.01)
Patent
CA 2393084
The present invention provides immunogens for generating anti-neonicotinoid antibodies as well as antibodies, methods, reagents, and kits for determining the presence of one or more neonicotinoid insecticides in a sample by immunoassay. The present invention has particular application to determination of the concentration of neonicotinoid insecticides applied to a plant propagation materials such as seed, said antibody is selective for a compound of formula (I) wherein A is 2-chloropyrid-5-yl or 2-chlorothiazol-5-yl; R and R3 independently are hydrogen or C1-C4alkyl; R1 and R2 independently are hydrogen or C1-C4alkyl or R1 and R2 are taken together with the nitrogen atoms to which they are attached to form an imidazoline or an oxadiazine ring; and X is N-NO2 or N-CN. Also claimed is a compound of formula (IA) and protein conjugate of formula (II) in which PR is a protein residue of a carrier molecule selected from the group consisting of a purified protein derivative (PPD, Tuberculin) from Diptheria virus, bovine serum albumin, cationized bovine serum albumin, human serum albumin, ovalbumin and keyhole limpet hemocyanin; or an enzyme residue selected from the group consisting of alkaline phosphatase, horseradish peroxidase, and .beta.-galactosidase.
L'invention concerne des immunogènes pouvant produire des anticorps anti-néonicotinoïdes, ainsi que des anticorps, des méthodes, des réactifs et des nécessaires pouvant déterminer par dosage immunologique la présence d'un ou de plusieurs insecticides néonicotinoïdes dans un prélèvement. L'objet de l'invention est particulièrement indiqué pour déterminer la concentration d'insecticides néonicotinoïdes appliqués sur sur des matériels de propagation de plantes, tels que des graines.
Brady James Francis
Simmons Dana Philip
Wilson Timothy Edward
Fetherstonhaugh & Co.
Syngenta Participations Ag
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