Process and device for specific binding assay

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 33/558 (2006.01) G01N 33/76 (2006.01)

Patent

CA 2074752

ABSTRACT OF THE DISCLOSURE A specific binding assay process which is useful for quick qualitative or quantitative measurement and can be used for various purposes, and a specific binding assay device suitable for the practice of the process, in which a substance to be assayed in a liquid test sample is determined qualitatively or quantitatively by developing the substance in a liquid test sample in a matrix, which comprises the steps of for example: allowing the substance to be assayed to react with a specific binding substance which has specific affinity for the substance to be assayed; allowing a signal substance generator (a substance which competes with the substance to be assayed for the specific binding substance and which generates a signal) to change its distribution in the matrix in response to the reaction of the above step, and; detecting resulting distributional changes at a detection means as changes of signals which are rate-limited by the mass transfer of a signal substance generated from the signal substance generator.

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