Plant-optimized polynucleotides encoding approximately 15...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/32 (2006.01) A01N 63/02 (2006.01) A01P 7/04 (2006.01) C07K 14/325 (2006.01) C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2703161

The subject invention provides new, plant-optimized polynucleotide sequences that encode pesticidal toxins. The polynucleotide sequences of the subject invention have certain modifications, compared to wild-type sequences, for example, that make them particularly well-suited for optimized expression in plants. Using the polynucleotide sequences described herein, the transformation of plants can be accomplished, using techniques known to those skilled in the art, in order to confer pest resistance upon said plants. In preferred embodiments, the subject invention provides plant-optimized polynucleotide sequences which encode approximately 15 kDa and approximately 45 kDa pesticidal proteins.

La présente invention a pour objet de nouvelles séquences de polynucléotides optimisées pour des plantes, qui codent des toxines pesticides. Les séquences de polynucléotides de la présente invention comportent certaines modifications comparativement aux séquences de type sauvage qui, par exemple, les rendent particulièrement bien adaptées pour une expression optimisée dans les plantes. Au moyen des séquences de polynucléotides décrites, la transformation de plantes peut être réalisée au moyen de techniques connues des experts en la matière, afin de leur conférer une résistance aux parasites. Dans les réalisations préférées, la présente invention permet d'obtenir des séquences de polynucléotides optimisées pour des plantes qui codent environ 15 kDa et environ 45 kDa de protéines pesticides.

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