Thermostable xylanases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) A23K 1/165 (2006.01) C12N 9/24 (2006.01)

Patent

CA 2385245

The present invention is directed to thermostable xylanase enzymes that are suitable for feed pelleting applications. The novel xylanase enzymes comprise at least 40 % of their optimal activity from a pH range from about pH 3.5 to about pH 6.0, and from about 40 to about 60 ~C, and exhibit at least 30 % of their optimal activity after a pre-incubation step for 30 minutes at 70 ~C in the presence of 40 % glycerol. Also disclosed are modified xylanase molecules comprising either a basic amino acid at position 162 (TrX numbering), or its equivalent position in other xylanase molecules, at least one disulfide bridge, or a combination thereof. The thermostable xylanase molecules of the present invention have a physiological temperature and pH optima and are useful as animal feeds additives since they can withstand the heat associated with feed sterilization and pellet formation, yet they exhibit optimal activity within an animal to aid in breakdown of ingested feed.

La présente invention concerne des enzymes xylanase thermostables, qui conviennent aux applications de granulation d'aliments pour animaux. Ces enzymes xylanase présentent au moins 40 % de leur activité optimale pour un pH compris entre 3,5 et 6,0, et entre environ 40 et 60 ·C, et au moins 30 % de leur activité optimale après une étape de pré-incubation de 30 minutes à 70 ·C en présence de 40 % de glycérol. L'invention concerne également des molécules de xylanase modifiées comprenant soit un acide aminé basique à la position 162 (numérotation TrX), ou une position équivalente dans d'autres molécules xylanase, au moins un pont disulfure, ou une combinaison de ceux-ci. Les molécules xylanase thermostables de l'invention présentent une température physiologique et un pH optimaux et sont utiles en tant qu'additifs d'aliments pour animaux car elles résistent à la chaleur associée à la stérilisation desdits aliments et à la granulation, tout en présentant une activité optimale dans la dissociation des aliments ingérés par un animal.

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