Method and apparatus for fluorescence pulse area/peak size...

Details Method and apparatus for fluorescence pulse area/peak size... Method and apparatus for fluorescence pulse area/peak size...

G - Physics

01

N

G01N 33/554 (2006.01) G01N 15/02 (2006.01) G01N 15/14 (2006.01) G01N 21/64 (2006.01) G01N 33/49 (2006.01) G01N 15/00 (2006.01) G01N 15/10 (2006.01)

Patent

CA 2104156

METHOD AND APPARATUS FOR FLUORESCENE PULSE AREA/PEAK SIZE PARAMETER MEASUREMENT FOR CELL ANALYSIS USING WHOLE BLOOD ABSTRACT A flow cytometry device is disclosed. The flow cytometry device has equipment for discriminating between particles of different sizes. The flow cytometry device detects fluorescence emitted by the particles and produces electrical pulses in response to the passage of the particles through a light beam. Electronic circuitry integrates the pulses. The integrated pulses are divided by the pulse amplitudes by subtraction of the logs of the integrals and pulse amplitudes to obtain a size parameter. The device has means to discriminate between particles having different size parameters.

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