Method for detecting sepsis

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/28 (2006.01) C12Q 1/04 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) G01N 30/02 (2006.01) G01N 30/72 (2006.01)

Patent

CA 2460130

The disclosure describes a method for detecting conditions indicative of sepsis. In one embodiment of the invention, an increase in the level of 3- chlorotyrosine or 3-bromotyrosine from the normal level in a sample of body fluid or tissue is indicative of early sepsis or infection. In another embodiment of the invention, the level of 3-chlorotyrosine or 3-bromotyrosine is measured or monitored to determine the response to therapeutic treatment of the infective condition in which a reductuion in the level that existed prior to the treatment is an early sign or indication that the treatment is working in vivo. In a preferred embodiment, the method of the invention is illustrated in a clinically relevant mouse model of sepsis.

L'invention concerne une méthode permettant de détecter des états révélateurs d'une sepsie. Selon un mode de réalisation, une augmentation du taux de 3-chlorotyrosine ou de 3-bromotyrosine par rapport au taux normal, dans un échantillon de liquide organique ou de tissu, est révélatrice d'un début de sepsie ou d'infection. Selon un autre mode de réalisation, le taux de 3-chlorotyrosine ou de 3-bromotyrosine est mesuré ou contrôlé pour déterminer la réponse au traitement thérapeutique de l'état infectieux pour lequel une réduction du taux mesuré avant le traitement est un signe ou une indication précoce que le traitement fonctionne in vivo. Dans un mode de réalisation préféré, la méthode selon l'invention est illustrée par un modèle de sepsie de souris pertinent du point de vue clinique.

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