Process for enriching for helicobacter pylori neutrophil...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) A61K 39/106 (2006.01) C12N 1/20 (2006.01) G01N 30/02 (2006.01) G01N 33/569 (2006.01) G01N 30/96 (2006.01)

Patent

CA 2326413

The invention relates to a process for enriching the presence of H.pylori NAP in a mixture or proteins, comprising the step of salting-out other proteins. NAP has been found to remain soluble at ammonium sulphate concentrations of 80 % and above. The process preferably comprises the further step of metal-chelate chromatography. The combination of salting-out and metal-chelate chromatography results in very pure NAP. The NAP preferably has the same sequence as NAP as naturally occurring in H.pylori and is free from sequences typically associated with recombinant protein production. The processes and NAP of the invention can be used in diagnostic and therapeutic products and processes.

L'invention concerne un procédé permettant d'augmenter la teneur en protéines activatrices des neutrophiles (NAP) de H.pylori d'un mélange de protéines, ledit procédé comprenant une étape de relargage des autres protéines. On a découvert que la NAP reste soluble à des concentrations de sulfates d'ammonium supérieures ou égales à 80 %. Le procédé comprend, de préférence, une étape supplémentaire de chromatographie à chélation métallique. La combinaison du relargage et de la chromatographie à chélation métallique permet d'obtenir une NAP de très haute pureté. La NAP comporte, de préférence, la même séquence que la NAP que l'on trouve à l'état naturel dans H.pylori, et elle est dépourvue des séquences généralement associées à la production de protéines recombinantes. Les procédés et la NAP de l'invention peuvent être utilisés dans des produits et des procédés diagnostiques et thérapeutiques.

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