Reading frame independent epitope tagging

Details Reading frame independent epitope tagging Reading frame independent epitope tagging

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) A61K 38/00 (2006.01) C07H 21/04 (2006.01) C07K 14/415 (2006.01) C07K 16/00 (2006.01) C12N 1/20 (2006.01) C12N 5/00 (2006.01) C12N 5/02 (2006.01) C12N 5/06 (2006.01) C12N 5/10 (2006.01) C12N 5/16 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/62 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2274986

Oligonucleotide sequence comprising a repeating nucleotide sequence encoding circularly permuted epitope tag, and vectors comprising the oligonucleotide sequences. Methods for using the sequences to tag proteins. Antibodies specific for the epitopes. Methods for detecting and purifiying proteins.

L'invention concerne une séquence d'oligonucléotides comprenant une séquence de nucléotides répétée codant un marqueur d'épitope à permutation circulaire, ainsi que des vecteurs comprenant les séquences d'oligonucléotides. L'invention concerne également des méthodes d'utilisation des séquences afin de marquer des protéines, des anticorps spécifiques aux épitopes ainsi que des méthodes de détection et de purification de protéines.

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