Marker free transgenic plants: engineering the chloroplast...

A - Human Necessities – 01 – H

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A01H 5/00 (2006.01) C12N 5/04 (2006.01) C12N 15/29 (2006.01) C12N 15/31 (2006.01) C12N 15/52 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2401954

The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting an antibiotic-free phytotoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation.

La présente invention concerne un procédé permettant de contourner le problème de l'utilisation de marqueurs sélectionnables résistants aux antibiotiques. En particulier, des plantes cibles sont transformées à l'aide d'un vecteur de plastes contenant des séquences d'ADN hétérologue codant une enzyme ou une protéine de détoxification de phytotoxine. Le procédé de sélection consiste à transformer un agent phytotoxique sans antibiotiques à l'aide de l'enzyme ou de la protéine de détoxification de phytotoxine exprimée afin d'obtenir un composé non toxique. L'invention concerne de nombreux procédés d'utilisation de la sélection sans antibiotiques dans la transformation chloroplastique.

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