Patterned cell network substrate interface and methods and...

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 1/14 (2006.01) C12M 1/34 (2006.01) C12N 5/00 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2565855

There is provided herein a method and apparatus suitable for use in studying cell membrane related activities. Activities of interest include patch-clamp related studies of networks of cells on a solid substrate. Cells are grown, preferably in a patterned manner, on a substrate having microholes therein. Seals between the cells and the microholes are formed. Each microhole is attached to a channel. In many cases only one hole will be attached to a single channel, allowing examination of effects of a stimulus at a number of different points in a network of one or more cell types. This may be interest, for example, to those wishing to study interactions between neurons or neuromuscular junctions.

La présente invention a trait à un procédé et un appareil appropriés pour une utilisation dans l'étude d'activités liées à la membrane cellulaire. Des activités d'intérêt comprennent des études liées à la méthode patch-clamp de réseaux de cellules sur un substrat solide. On réalise la croissance de cellules, de préférence en configuration à motif, sur un substrat présentant des micro-trous. On forme des joints entre les cellules et les micro-trous. Chaque micro-trou est fixé à un canal. Dans plusieurs cas un seul trou va être fixé à un canal unique, permettant l'examen des effets d'un stimulus à une pluralité de points différents dans le réseau d'un ou de plusieurs types de cellules. L'invention peut présenter un intérêt, par exemple, pour ceux désirant étudier des interactions entre neurones ou entre jonctions neuromusculaires.

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