Elongated membrane flow-through diagnostic device and method

Details Elongated membrane flow-through diagnostic device and method Elongated membrane flow-through diagnostic device and method

G - Physics

01

N

167/44

G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01) G01N 37/00 (2006.01)

Patent

CA 2017329

ABSTRACT An assay device and method for detecting an analyte in a liquid sample derived from biological specimen (e.g. urine or serum). The device includes (a) a porous reaction membrane with an immobilized receptor which is capable of binding to the target substance, and (b) a body of absorbent material located adjacent to the porous reaction membrane which is capable of absorbing the liquid sample. The device also includes an elongate fluid port with a fluid seal disposed at the periphery of the fluid port defining a seal between a container wall and reaction membrane. Preferably, the reaction membrane is a strip and the fluid seal means is a continuous rim projecting from the container wall towards the strip. In one embodiment, the receptor is an antigen, preferably a protein blot (e.g. HIV), and the bindable target substance is antibody. A blocking solution (preferably 1-2% Tween-20 surfactant and BSA is applied to the membrane before sample addition.

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