Method of protein production using mitochondrial translation...

C - Chemistry – Metallurgy – 12 – N

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C12N 7/04 (2006.01) A61K 39/12 (2006.01) C07K 14/005 (2006.01) C07K 14/02 (2006.01) C07K 14/10 (2006.01) C07K 14/18 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2244709

A method of producing viral antigens in vitro by infecting animal organ tissue rich in mitochondria with a virus, including human hepatitis B virus (HBV), and culturing the infected tissue in vitro is disclosed. A method of producing proteins in vitro by transfecting mitochondria-rich animal tissue with a recombinant HBV-based vector and culturing the transfected tissue in a dynamic tissue culture system is disclosed.

L'invention a trait à un technique de production d'antigènes viraux in vitro consistant à infecter un tissu d'organe appartenant à un animal, riche en mitochondries, à l'aide d'un virus, y compris le virus de l'hépatite B humain (VHB) et à mettre en culture le tissu infecté in vitro. L'invention porte également sur un procédé de production de protéines in vitro par transfection d'un tissu animal riche en mitochondries à l'aide d'un vecteur de recombinaison issu du VHB, ce tissu transfecté étant ensuite mis en culture dans un système dynamique de culture tissulaire.

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