Improved expression of cry3b insecticidal protein in plants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/325 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2340324

The present invention discloses methods and compositions comprising a group of novel expression cassettes which provide significantly improved levels of accumulation of Coleopteran inhibitory Cry3B and Cry3B variant amino acid sequences when these are expressed in plants. The preferred embodiments of the invention provide at least up to ten fold higher levels of insect controlling protein relative to the highest levels obtained using prior compositions. In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.

L'invention concerne des procédés et des compositions comprenant un groupe de nouvelles cassettes d'expression qui assurent des niveaux sensiblement plus élevés d'accumulation des séquences d'acides aminés de Cry3B et des variantes de Cry3B, qui ont un effet inhibiteur sur les coléoptères, lorsque ces séquences sont exprimées dans des plantes. Dans les modes de réalisation préférés, on obtient des quantités de protéines repoussant les insectes qui sont au moins dix fois supérieures aux quantités les plus élevées obtenues avec les compositions existantes. On notera tout particulièrement le maïs transgénique exprimant des niveaux élevés d'une protéine conçue pour manifester une plus grande toxicité envers les coléoptères: il offre une meilleure protection contre les insectes et présente moins de risques de favoriser le développement de populations d'insectes cibles qui résisteraient à la protéine à action insecticide.

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