Sequence based screening

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2375082

Provided is a method of obtaining a nucleic acid profile of a sample. The method includes creating a DNA library from a plurality of nucleic acid sequences of a mixed population of organisms and sequencing at least one clone in the DNA library. The sequence is compared to a database and identifying sequences in the database which have homology to a clone in the library thereby obtaining a nucleic acid profile of the mixed population of organisms.

L'invention porte sur un procédé visant à obtenir un profil d'acide nucléique d'un échantillon. Ce procédé consiste à créer une banque d'ADN à partir d'une pluralité de séquences d'acide nucléique d'une population mixte d'organismes et à séquencer au moins un clone dans la banque d'ADN. La séquence est ensuite comparée à une base de données, et on identifie des séquences de la base de données qui ont une homologie avec un clone de la banque d'ADN, ce qui permet d'obtenir un profil d'acide nucléique de la population mixte d'organismes.

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