Variants of vascular endothelial cell growth factor, their...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/18 (2006.01) A61K 38/18 (2006.01) C07K 14/475 (2006.01) C07K 14/52 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2230144

The present invention involves the preparation of vascular endothelial growth factor (VEGF) variants which provide materials that are selective in respect of binding characteristics to the kinase domain region and the FMS-like tyrosine-kinase region, respectively KDR and FLT-1. The respective KDR and FLT- 1 receptors are bound by corresponding domains within the VEGF compound domains. The variants hereof define those two binding regions and modify them so as to introduce changes that interrupt the binding to the respective domain. In this fashion the final biological characteristics of the VEGF molecule are selectively modified.

La présente invention se rapporte à la préparation de variants du facteur de croissance des cellules de l'endothélium vasculaire (VEGF), lesdits variants constituant des matières sélectives vis à vis des caractéristiques de liaison à la région du domaine kinase (KDR) et à la région tyrosine kinase de type protéine FMS (FLT-1). Les récepteurs respectifs des régions KDR et FLT-1 sont liés par des domaines correspondants à l'intérieur des domaines composés du VEGF. Les variants de ce facteur définissent ces deux régions de liaison et les modifient de telle sorte que les modifications introduites interrompent la liaison au domaine respectif. Il est ainsi possible de modifier sélectivement les caractéristiques biologiques finales de la molécule du facteur VEGF.

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