High throughput dna sequencing vector

Details High throughput dna sequencing vector High throughput dna sequencing vector

C - Chemistry, Metallurgy

12

N

C12N 15/64 (2006.01) C12N 9/38 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12N 15/69 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2321592

High throughput DNA sequencing vectors for generating nested deletions using enzymatic techniques and/or transposition-based techniques are disclosed. Methods of constructing contigs of long DNA sequences and methods of generating nested deletions are also disclosed. A truncated lacZ derivative useful in measuring the copy number of the lacZ derivative in a host cell is also disclosed.

L'invention concerne des vecteurs de séquençage d'ADN à haut rendement permettant de réaliser des délétions emboîtées à l'aide de techniques enzymatiques et/ou de techniques basées sur la transposition. L'invention concerne également des méthodes permettant de construire des contigs de longues séquences d'ADN et des méthodes permettant de générer des délétions emboîtées. L'invention concerne enfin un dérivé tronqué du gène lacZ utile pour mesurer le nombre de copies du dérivé du gène lacZ dans une cellule hôte.

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