Method for imaging on thin solid-state interface between two...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12M 1/34 (2006.01) G01N 15/10 (2006.01) G01N 21/03 (2006.01) G01N 21/77 (2006.01) G01N 33/483 (2006.01) G02B 21/26 (2006.01)

Patent

CA 2756233

Described herein is a fluid cell for an optical microscopy tool having a solid state membrane having a first side and a second, opposing side; a first fluid chamber comprising a first fluid having a first refractive index located on the first side of the membrane; and, a second fluid chamber comprising a second fluid having a second refractive index located on the second side of the membrane, the second refractive index being different than the first refractive index. Also described herein is a method for imaging a single biomolecule, the method including generating a field of evanescent illumination at a solid state membrane between a first fluid and a second fluid having different refractive indexes; and detecting light emitted by optical detectors linked to the single biomolecules at the solid state membrane.

L'invention porte sur une cellule de fluide pour un outil de microscopie optique ayant une membrane à l'état solide ayant un premier côté et un second côté opposé ; une première chambre de fluide comprenant un premier fluide ayant un premier indice de réfraction et située sur le premier côté de la membrane ; et une seconde chambre de fluide comprenant un second fluide ayant un second indice de réfraction et située sur le second côté de la membrane, le second indice de réfraction étant différent du premier indice de réfraction. L'invention porte également sur un procédé d'imagerie d'une biomolécule unique, le procédé comprenant la génération d'un champ d'éclairage évanescent au niveau d'une membrane à l'état solide entre un premier fluide et un second fluide ayant différents indices de réfraction ; et la détection d'une lumière émise par les détecteurs optiques liés aux biomolécules uniques au niveau de la membrane à l'état solide.

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