Production of 2s canola protein involving ion exchange

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/415 (2006.01) C07K 1/14 (2006.01) C07K 1/18 (2006.01) C11D 1/00 (2006.01)

Patent

CA 2693092

Substantially pure 2S canola protein is obtained substantially free from 7S and 12S canola protein by a procedure in which 2S canola protein is captured by binding to a cation-exchange medium while permitting other proteins and impurities to be washed away. The 2S canola protein then is removed from the cation-exchange medium by exposure of the cation-exchange medium to saline at a suitably high salt concentration.

L'invention propose un procédée permettant de production une protéine de canola 2S sensiblement pure et sensiblement exempte de protéine de canola 7S et 12S. Le procédé consiste à capturer la protéine de canola 2S par liaison à un milieu d'échange de cations tout éliminant d'autres protéines et impuretés par lavage ; retirer la protéine de canola 2S du milieu d'échange de cations par exposition du milieu d'échange de cations à une solution saline concentrée de façon appropriée.

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