Method and apparatus for measuring fluorescence in liquids

G - Physics – 01 – N

Patent

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G01N 33/28 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2750404

The invention concerns a method of tagging a bulk material with a fluorescent marker compound and subsequently identifying the fluorescent compound by measuring its fluorescent emission, wherein the fluorescence of the tagged sample is measured before and after quenching the fluorescent compound in order to correct the measured fluorescence for the effect of the inherent fluorescent properties of the bulk material itself. The measurement may be further corrected to account for the absorbance of the material which is also known to have an effect on the measured fluorescence. Apparatus for carrying out the method is also described.

L'invention concerne un procédé consistant à marquer une substance en vrac avec un composé fluorescent de marquage et à identifier le composé fluorescent grâce à la mesure de son émission de fluorescence. La fluorescence de l'échantillon marqué est mesurée avant et après la désactivation du composé fluorescent afin de prendre en compte les propriétés de fluorescence propres à la substance en vrac pour corriger la fluorescence mesurée. L'absorbance de la substance qui est également connu pour avoir un effet sur la fluorescence mesurée peut également être prise en compte pour corriger la mesure. L'invention concerne également un appareil pour mettre en uvre le procédé.

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