Laser desorption and ionization mass spectrometer with...

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B01D 59/44 (2006.01)

Patent

CA 2571459

Laser desorption/ionization time-of-flight mass spectrometer ("LDI-TOF-MS") devices, and methods, that accurately measure the mass of analytes contained in a sample and which also measure the quantities of analytes present in a sample in a consistent manner from instrument-to-instrument and over time on a single instrument. In particular, the invention provides LDI-TOF-MS devices and methods in which: 1) the energy of the laser pulse and the area of the sample illuminated (fluence) is consistent and controlled so as to produce consistent conditions for analyte desorption and ionization; 2) the mass analyzer behaves in a reproducible manner; and 3) the detection system produces a signal that consistently represents the arrival of ions of different masses.

Cette invention concerne des dispositif et des procédés à spectromètre de masse à temps de vol, à ionisation/désorption laser ("LDI-TOF-MS") permettant de mesurer avec précision des quantités de substances à analyser présentes dans un échantillon de manière fidèle, d'un instrument à un autre et dans le temps sur un instrument unique. En particulier, l'invention concerne des dispositifs et des procédés LDI-TOF-MS dans lesquels: 1) l'énergie de l'impulsion laser et la zone éclairée de l'échantillon (fluence) est régulière et commandée de manière à produire des conditions uniformes pour la désorption et l'ionisation des substances à analyser; 2) l'analyseur de masse se comporte de manière reproductible; et 3) le système de détection produit un signal qui représente régulièrement l'arrivée d'ions de masses différentes.

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