Recombinant dna and transformant containing the same

Details Recombinant dna and transformant containing the same Recombinant dna and transformant containing the same

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C07K 14/505 (2006.01) C07K 14/56 (2006.01) C12N 5/10 (2006.01) C12N 15/16 (2006.01) C12N 15/23 (2006.01)

Patent

CA 2137120

Insertion of IFN-alpha promoters in recombinant DNAs improves their expression efficiencies for useful polypeptides. Expression of such a recombinant DNA in host cells of mammalian origin is artificially controllable by the presence and absence of external stimuli using IFN-alpha inducers. Thus trans- formants with such a recombiant DNA readily increase to a miximized cell density with causing neither damages nor extinc- tion due to polypeptides they produce, and subsequent exposure to IFN-alpha inducers allows the proliferated cells to effi- ciently produce polypeptids with significant glycosylations.

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