Double antibody assay by precipitation of a colored protein

G - Physics – 01 – N

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167/40

G01N 33/532 (2006.01)

Patent

CA 1128416

METHODS AND KITS FOR DOUBLE ANTIBODY IMMUNOASSAY PROVIDING A COLORED PELLET FOR EASY VISUALIZATION Abstract of the Disclosure Methods and kits for performing a double antibody immunoassay are described. The immunoassay comprises: reacting an antigen and a labelled version of said antigen with a first antibody against said antigen and said first antibody with a second antibody against said first antibody to precipitate a complex of said antigen, said first antibody and said second antibody, and precipitating a colored protein to form a precipitate comprised of said complex and colored protein. In a preferred embodiment, the first and second antibodies can be prereacted to simplify the assay procedure.

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