Analytical determination of lipase

Details Analytical determination of lipase Analytical determination of lipase

C - Chemistry, Metallurgy

12

Q

150/3.2

C12Q 1/34 (2006.01) G01N 21/25 (2006.01)

Patent

CA 1120833

ABSTRACT OF THE DISCLOSURE A test for lipase activity is provided wherein trilinolein, or an equivalent as defined herein, is employed in an emulsified form as a substrate. Reduced incubation times and improved accuracy are obtained by providing an excess of lipoxygenase to convert the linoleic acid product of lipolysis to linoleic acid hydroperoxide which is measur- able with great accuracy at low concentrations by spectro- photometric or kinetic assay techniques, for example.

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