Single sample method for determination of lipoprotein...

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150/11, 150/3

G01N 33/48 (2006.01) C12Q 1/28 (2006.01) G01N 31/02 (2006.01) G01N 33/92 (2006.01)

Patent

CA 1086197

Abstract of the Disclosure A single-sample method for determining concentrations of individual lipoprotein classes and lipids in blood by turbidimetric measurement. The blood serum is mixed with an ionic-strength-sensitive agent for forming an insoluble complex with the chylomicron, very low density, and low density lipoprotein classes, and the ionic strength of the mixture is raised in steps to cause progressive dissolution of each class of complex from that of highest density lipoprotein to that of lowest density lipoprotein. Measurement of the turbidity due to the insoluble complex(es) present at each step allows calculation of the turbidity due to the complex of each individual lipoprotein class and from that the concentration of each lipoprotein class and lipid in the blood sample.

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