Antibiotic detection method

Details Antibiotic detection method Antibiotic detection method

C - Chemistry, Metallurgy

12

Q

150/16.1

C12Q 1/02 (2006.01) C12Q 1/04 (2006.01) C12Q 1/18 (2006.01) F04B 39/12 (2006.01) G01N 33/02 (2006.01) G01N 33/487 (2006.01)

Patent

CA 1108516

ABSTRACT OF THE DISCLOSURE A process for rapidly detecting as little as .001 I.U. of antibiotic in a sample of liquid such as milk. The process comprises the steps of incubating the sample together with a tagged antibiotic or antibiotic precursor and antibiotic sensitive cells under conditions which allow antibiotic molecules to attach to receptor sites in or on the cells, separating the cells with immobilized antibiotic from the remainder of the reaction mixture, and determining the quantity of tagged antibiotic on the cells. The amount of tagged antibiotic on the cells is a function of the quantity of antibiotic present in the sample.

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