Stabilized liquid enzyme and coenzyme compositions and...

C - Chemistry – Metallurgy – 12 – Q

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150/3.3

C12Q 1/00 (2006.01)

Patent

CA 1127055

ABSTRACT OF THE DISCLOSURE Labile coenzymes, such as nicotinamide-adenine dinucleotide (NAD), are stabilized by treatment with an organic solvent, such as propylene glycol, in an aqueous media and a suitable polymer which does not inhibit enzymatic activity. An azide compound may be added to the solution, which not only serves as a bacterio- stat, but also functions as a stabilizer. In addition, a second coenzyme, such as adenosine triphosphate (ATP), may also be stabilized in the same solution. Moreover, one or more enzymes, such as hexokinase and glucose-6-phosphase dehydrogenase, may be stabilized against denaturation in the same solution. The composition exhibits excellent shelf life, and the container therefor may be repeatedly opened for use without any substantial degradation of the labile components. Moreover, all of the components may be packaged in a single solution. -1-

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