Method and apparatus for detection and purification of...

Details Method and apparatus for detection and purification of... Method and apparatus for detection and purification of...

G - Physics

01

N

167/37

G01N 21/01 (2006.01) G01N 33/48 (2006.01) G01N 33/553 (2006.01)

Patent

CA 1047918

Abstract of the Disclosure Method and apparatus for the detection and purification of proteins and antibodies based upon the effect that any arbitrary protein will adsorb onto a substrate in a monomolecular layer only, but that a specific antibody (or antigen) for such arbitrary protein will bond thereto to from a bi-molecular protein layer on the substrate is disclosed. In practicing this invention, a first layer of protein is adsorbed onto a substrate and the coated substrate is then exposed to a solution suspected of containing the specific antibody (or antigen) of interest. The substrate is then examined to determine whether a monomolecular or bi- molecular layer of protein is adhering thereon. Optical, electrical, And chemical means for examining the coated substrate are disclosed. Purification is provided by severing the immunological bond with weak acid.

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