Hemolytic method for the continuous determination of...

G - Physics – 01 – N

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167/37, 73/59

G01N 33/96 (2006.01) G01N 33/555 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1162846

HEMOLYTIC METHOD FOR THE CONTINUOUS DETERMINATION OF ANTISTREPTOLYSIN O ANTIBODIES IN BLOOD OR SERUM SAMPLES, USING OXIDISED STREPTOLYSIN O ABSTRACT The hemolytic method for the continuous determination of antistreptolysin O antibodies (ASO) in blood samples consists of reacting a first reagent containing a single dose of oxidised SO with the specific antibodies which may be present in the blood sample under examination, allowing the necessary time to pass for the reaction between the oxidised SO and said antibodies to take place, returning the oxidised SO to its reduced state by adding a second reagent, and measuring the rate of hemolysis. The continuous determination of the ASO titre is obtained by comparing said rate of hemolysis with the rate of hemoly- sis shown graphically for samples of known ASO titre. - 1 -

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