G - Physics – 01 – N
Patent
G - Physics
01
N
167/45
G01N 33/554 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/74 (2006.01) G01N 33/88 (2006.01)
Patent
CA 1105381
IN THE UNITED STATES PATENT OFFICE Abstract-of the Disclosure: According to the present invention there is provided a sensitive quantitative assay for the determination of a hapten "X" which comprises: preparing a conjugate of the hapten X which conjugate is adapted to ellicit antibody formation, and injecting a mammal with said conjugate, resulting in a specific antibody against the said hapten, "anti-X"; preparing a conjugate of the hapten X and of another entity Y, which is either another hapten or a larger molecule, injecting a mammal with the entity Y if it is a larger molecule, or with a conjugate thereof if it is a small molecule which does not by itself ellicit antibody formation, so as to form anti-Y antibodies, preparing a conjugate of a suitable enzyme and the antibody against Y, i.e. an (enzyme)-(anti-Y)-conjugate; adsorbing the antibodies against X, i.e. anti-X on a solid support, conitacting said solid support with a mixture containing the unknown quantity of hapten X and a known quantity of the X-Y conjugate; removing unreacted X and X-Y, leaving all anti-X sites occupied, adding enzyme-labelled anti-Y antibody; adding a suitable substrate resulting in a color reacting and measuring the intensity of color indicating the quantity of bound enzyme and deducing from calibration curves the quantity of the hapten X; and to a kit for carrying out such assay.
299085
Hexter Charles S.
Sadeh Dora
Kirby Eades Gale Baker
Miles-Yeda Ltd.
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