C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/145, 195/46
C12N 5/00 (2006.01) C12M 3/04 (2006.01) C12N 1/10 (2006.01)
Patent
CA 1081149
ABSTRACT OF THE DISCLOSURE A method for the mass growth of diploid cells is characterized by the introduction of the cell suspension, the nutrient medium or other solution into a system of communicat- ing chambers via a central supply channel. The communicating chambers are formed by stacking parallel flat troughs, whose bottoms are arranged substantially vertically and distributed in accordance with the principle of communicating tubes. The air pressure is equalized via a central aeration and exhaust channel located in the upper part of the system of chambers. The supply and aeration or exhaust lines are closed and the system is moved to the horizontal position thereby distributing the cell suspension, nutrient medium, or other solution over the useful surface of the substantially horizontal troughs and effecting the growth of the cells. Upon completion of the growth the system of chambers is restored to the vertical position, and the cell suspension or cell product is recovered.
279325
Pakos Valdemar
Skoda Rastislav
LandOfFree
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