Biological cell analyzing system

Details Biological cell analyzing system Biological cell analyzing system

G - Physics

01

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73/59

G01N 33/48 (2006.01) A61B 5/145 (2006.01) G01N 15/14 (2006.01) G06F 19/00 (2006.01)

Patent

CA 1036385

ABSTRACT A biological cell analyzing system which is capable of automatically categorizing unstained biological cells as normal or non-normal. The cells are made to flow through a transparent tube in single file and are scanned with a mixture of ultra-violet and visible light. The cytoplasm and nucleus of each cell absorb ultra-violet radiation to different degrees, and the emergent light signal, as modulated by the scanned cells, is detected, amplified, and extended to a data processor which logically analyzes the signal from each cell on a real-time basis. The visible light signal is subtracted from the ultra- violet light signal to improve the signal/noise ratio of the latter, and to automatically cancel out non-biological debris. A number of acceptance tests are electronically performed on each cell, and if any of the tests is failed the cell is cate- gorized as non-normal. Ambiguous conditions, resulting for example from the clumping of cells, are identified and sepa- rately counted. The system can process up to several thousand cells from a single sample during a one-minute run.

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