Method for preparation of antihemophilic factor

Details Method for preparation of antihemophilic factor Method for preparation of antihemophilic factor

A - Human Necessities

61

K

195/39.1

A61K 35/16 (2006.01) C07K 14/755 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1086648

ABSTRACT OF THE DISCLOSURE An improved method for preparation of purified AHF is disclosed. The method includes the cryoprecipitation of plasma, followed by a cold extraction of the cryoprecipitate at low temperature, approximately 0°C, to obtain a cold insoluble fraction. The cold insoluble fraction is then extracted at approximately 21°C to obtain an AHF-containing solution. By incorporating a cold extraction step to selectively remove some impurities prior to extraction of AHF from cryoprecipitate, there has been obtained a substantial increase in the purity as well as the potency of AHF without the use of any new precipitant or adsorbent in the system.

282972

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