Process for kinetic determination of substrate and reagent...

Details Process for kinetic determination of substrate and reagent... Process for kinetic determination of substrate and reagent...

C - Chemistry, Metallurgy

12

Q

150/15, 150/2, 1

C12Q 1/00 (2006.01) C12Q 1/25 (2006.01) C12Q 1/54 (2006.01) C12Q 1/60 (2006.01) C12Q 1/61 (2006.01) G01N 33/66 (2006.01)

Patent

CA 1100022

ABSTRACT OF THE DISCLOSURE A reagent and method for the kinetic determination of enzyme substrates by means of coupled enzymatic reactions which reagent comprises such enzymes and reaction components in amounts such that the most specific part reaction of the reaction sequence taking place upon the addition of the substrate to an aqueous solution of the reagent is rate- determining for the whole of the reaction sequence and takes place according to the first or pseudo-first order the reagent and method can be employed in automatic analysers for rapid analysis for determination of a substrate.

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