Anucleated live e. coli vaccine

Details Anucleated live e. coli vaccine Anucleated live e. coli vaccine

C - Chemistry, Metallurgy

12

N

167/135, 195/49

C12N 15/00 (2006.01) A61K 39/02 (2006.01) A61K 39/108 (2006.01)

Patent

CA 1146882

ABSTRACT OF THE DISCLOSURE An E. coil strain producing metabolically active but non-reproductive, anucleated live cells, which contain the K99 surface antigen, is obtained by transferring the K99 plasmid from a K99+ entero pathogenic E. coli strain into an E. coli strain which generates progeny of cells that lack the ability to multiply. From the resulting E. coli strain a "live" vaccine is prepared. Separation and purification of the anucleated live cells from growing cells is the basis for the "live" and chemically and/or physically unmodified vaccine. The vaccine induces the production of antibodies against growing and infective enteropathogenic K99+ E. coli in cattle and is, thus effective against coliform enteritis.

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