Controlled protein fractionation

Details Controlled protein fractionation Controlled protein fractionation

C - Chemistry, Metallurgy

07

K

182/15.3

C07K 14/765 (2006.01) C07K 1/30 (2006.01)

Patent

CA 1161368

ABSTRACT OF THE DISCLOSURE: A controlled protein fractionation method in which a cold protein-containing solution is circulated in contact with one side of a membrane and the temperature is controlled by circu- lating a protein precipitant solution in contact with the oppo- site side of the membrane. The flow rate is increased and back pressure is exerted on the precipitant solution to cause it to penetrate the membrane, mix with the protein solution and pre- cipitate protein in fine particles along an inside membrane wall. The precipitated protein may be concentrated and separated by centrifugation or diafiltration. The membrane is preferably in the form of a plurality of hollow fibers. Control of process variables increases yield and purity, decreases processing time and reduces degree of denaturization. The method is especially applicable to carrying out the Cohn cold alcohol fractionation process.

351044

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